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KMID : 0878220160280020147
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Journal of Sasang Constitutional Medicine
2016 Volume.28 No. 2 p.147 ~ p.162
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A Study of the Effect on Obesity in Taeeumin Animal-experimental Model Induced Lung Fibrosis with Bleomycin
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Kim Yoon-Ha
Park Jung-Hwan
Kwak Jin-Young
Park Jung-Mi
Ahn Taek-Won
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Abstract
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Objectives: The objective of this study is to develop a taeeumin animal-experimental model induced lung fibrosis with Bleomycin
and evaluate the effect on obesity in this animal-experimental model.
Methods: The subjects were divided into 3 groups : normal group, high fat diet(HFD) control group, and HFD group
administered with bleomycin(n=10 per group). To develop taeeumin animal-experimental model with reduced
respiratory metabolism, 8-week-old C57BL/6 mice were administered with 0.03ml solution of bleomycin 1U/ml
dissolved in distilled water, intratracheal(IT), once. Then, the HFD control group and the experimental group
were fed with high fat diet for 6 weeks. Airway hyperresponsiveness(AHR) to methacholine was measured at
the 1st and 3rd week after bleomycin was administered. Food intake and body weight were measured at regular
time weekly. After the final experiment, blood was gathered by cardiac puncture for bloodchemical examination
and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed.
Results and Conclusions: Through the experiment, it was found that Bleomycin induced Taeeumin animal-experimental models have leptin
resistace. In the experimental group administered with Bleomycin, fatty acid synthesizing gene expression increased
and energy metabolizing gene expression decreased. As mRNA expression of adiponectin decreased, it was found
that Taeeuim animal-experimental model is susceptible to metabolic syndrome and cardiovascular diseases.
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KEYWORD
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Taeeumin, Obesity, Leptin, Adiponectin, Bleomycin, Animal-experimental model
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